A universal karyotypic system for hexaploid and diploid Avena species brings oat cytogenetics into the genomics era

Abstract Background The identification of chromosomes among Avena species have been studied by C-banding and in situ hybridization. However, the complicated results from several cytogenetic nomenclatures for identifying oat are often contradictory. A universal karyotyping nomenclature system precise chromosome comparative evolutionary studies would be essential genus based on recently released genome sequences hexaploid diploid species. Results Tandem repetitive were predicted physically located chromosomal regions sativa OT3098 assembly v1. Eight new oligonucleotide (oligo) probes sequential fluorescence hybridization (FISH) designed then applied mitotic metaphase spreads A. brevis , nuda wiestii ventricosa fatua We established a high-resolution standard karyotype distinct FISH signals multiple oligo probes. painting with bulked oligos, wheat-barley collinear regions, was used to validate linkage group assignment individual chromosomes. integrated our Oligo-FISH earlier through methods, subsequently determined breakage points some translocations . Conclusions This will powerful tool describing genetic diversity, rearrangements relationships genomic approaches.